melatonin receptor 1b  (Novus Biologicals)

Journal: Experimental and Therapeutic Medicine

Article Title: Melatonin exerts neuroprotective effects in mice with spinal cord injury by activating the Nrf2/Keap1 signaling pathway via the MT2 receptor

doi: 10.3892/etm.2023.12325

Figure Lengend Snippet: Melatonin can upregulate the expression of the MT2 receptor and activate the Nrf2 signaling pathway. (A-D) Representative blots and quantification of the protein expression levels of MT2, Nrf2 and Keap1 were assessed in each experimental group. (E and F) mRNA levels of Nrf2 and Keap1 were measured in the spinal cord tissue of each group at 7 days post-injury. * P≤0.05. Nrf2, nuclear factor-erythroid 2-related factor 2; Mel, Melatonin; Veh, Vehicle; 4PP, 4-phenyl-2-propionamidotetralin.

Article Snippet: The membranes were then probed with specific primary antibodies and incubated at 4˚C in a refrigerator for 2 h, including MT2 (1:3,000; cat. no. NLS932; Novus Biologicals, LLC), Nrf2 (1:3,000; cat. no. A0674; ABclonal Biotech Co., Ltd.), Keap1 (1:3,000; cat. no. A17061; ABclonal Biotech Co., Ltd.), iNOS (1:3,000; cat. no. AF0199; Affinity Biosciences, Ltd.) and Arg1 (1:3,000; cat. no. DF6657; Affinity Biosciences, Ltd.); followed by incubation with a secondary Goat Anti-Rabbit IgG (H+L) HRP-conjugated antibody (1:3,000; cat. no. S0001; Affinity Biosciences, Ltd.) for 2 h. Protein bands were visualized using enhanced chemiluminescence (ECL) (cat. no. PE0010; Beijing Solarbio Science & Technology Co., Ltd.), and the intensity of the bands was quantified using ImageJ 1.54 software (National Institutes of Health).

Techniques: Expressing

Journal: PLOS ONE

Article Title: Expression of circadian regulatory genes is dysregulated by increased cytokine production in mice subjected to concomitant intestinal injury and parenteral nutrition

doi: 10.1371/journal.pone.0290385

Figure Lengend Snippet: Antibodies used for Western analysis in this study.

Article Snippet: Mtnr1b , Novus , NLS932.

Techniques: Western Blot